|
KMID : 0620920220540040483
|
|
Experimental & Molecular Medicine
2022 Volume.54 No. 4 p.483 ~ p.492
|
|
|
Single-cell RNA sequencing analysis of human bone-marrow-derived mesenchymal stem cells and functional subpopulation identification
|
|
Xie Zhongyu
Yu Wenhui
Ye Guiwen
Li Jinteng
Zheng Guan
Liu Wenjie
Lin Jiajie
Wang Peng
Wu Yanfeng
Shen Huiyong
|
|
|
Abstract
|
|
|
|
Mesenchymal stem cells (MSCs) are a common kind of multipotent cell in vivo, but their heterogeneity limits their further applications. To identify MSC subpopulations and clarify their relationships, we performed cell mapping of bone-marrow-derived MSCs through single-cell RNA (scRNA) sequencing. In our study, three main subpopulations, namely, the stemness subpopulation, functional subpopulation, and proliferative subpopulation, were identified using marker genes and further bioinformatic analyses. Developmental trajectory analysis showed that the stemness subpopulation was the root and then became either the functional subpopulation or the proliferative subpopulation. The functional subpopulation showed stronger immunoregulatory and osteogenic differentiation abilities but lower proliferation and adipogenic differentiation. MSCs at different passages or isolated from different donors exhibited distinct cell mapping profiles, which accounted for their corresponding different functions. This study provides new insight into the biological features and clinical use of MSCs at the single-cell level, which may contribute to expanding their application in the clinic.
|
|
|
KEYWORD
|
|
|
Mesenchymal stem cells, Transcriptomics
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
    
|